A study of soil nutrient hotspots was conducted by sampling two forest soils both destructively and with resin-based methods. In conjunction with the soil sampling, O horizon interflow collectors were established near each plot. Based on previous work, we hypothesized that nutrient hotspots existed in the soils and that they would be more common for less abundant than more abundant nutrients. We also hypothesized that nutrient-enriched O horizon interflow was occurring at these sites and could cause hotspots in where it enters the soil in preferential flow paths. The first hypothesis was only partially supported: hotspots, as indicated by significant positive skew and statistical outliers, occurred not only for least abundant nutrients, but for nearly all measured nutrients by various methods. The second hypothesis was supported: O horizon interflow did occur, was enriched in nutrients, and could well have caused some nutrient hotspots. We found one candidate for such a hotspot where extreme outliers for all but one H₂O-extractable nutrient was identified.