The efficiency with which tropical forage grasses (C4 metabolism) can use radiation and convert it into photoassimilates has long been recognized. This, however, is dependent on the photosynthetic rates of individual leaves and the patterns of canopy light interception. Detailed descriptions of these basic processes, including those involving the physiology of carbon assimilation in tropical forages and their responses to the environment are still scarce for many tropical genera, including Panicum spp. The objective of this study was to identify and define carbon uptake patterns on three leaf categories and on three leaf blade portions of Panicum spp grasses (Massai and Tobiatã), in response to irradiance levels. Maximum leaf photosynthesis rates were measured, and light response curves were fit to increasing levels of irradiance for five Panicum spp. genotypes (Tobiatã, Mombaça, Tanzânia, Atlas and Massai). Leaf photosynthesis was affected by leaf category on tiller and leaf blade portion. Among leaf categories, higher photosynthesis rate (20.6 μmol CO₂ m^-2 s^-1) was measured on the youngest fully expanded leaf, followed by the mature leaf and by the expanding leaf. Middle, and apical leaf sections had the highest photosynthesis rates (24.2 and 26.3 μmol CO₂ m^-2 s^-1, respectively). Tobiatã had highest photosynthesis rate (33.5 μmol CO₂ m^-2 s^-1) while Massai had the lowest (20.3 μmol CO₂ m^-2 s^-1). The two grasses had similar light curve parameters (P=0.7502 for maximum photosynthesis and P=0.6458 for quantum efficiency), indicating similar photosynthetic responses to light intensity across genotypes. Photosynthetic responses of Panicum grasses change with category and portion of the leaf blade, with highest photosynthesis rates measured on the middle portion of the youngest fully expanded leaf.