Miscanthus sinensis can propagate through seed. However, more research is needed to understand the physiology of these seeds.  Our objectives were to determine the optimal germination temperature requirements, and to develop a standard germination test protocol for the species.  Seed germination was studied using a two way thermogradient table.  The temperature gradient in the table had 30 cells with alternating day/night temperatures and 6 cells with constant temperatures.  Seeds exposed to alternating temperatures showed a greater germination rate than those at constant temperatures.  The highest germination percentage was recorded in cells with a temperature combination of 16°C for 16 h and 22°C for 8 h.  These results are important to seed analysts developing a standard germination protocol for Miscanthus sinensis seed.