This is to demonstrate the critical procedures for improving the accuracy and precision of chemical analyses for various samples in the Soil and Water Research Laboratory at UF TREC-Homestead certified by NELAC (national Environmental Laboratory of Accreditation Conference). Major challenges that most laboratories face for their credibility are quality assurance and quality control (QA/QC) procedures, which usually play a critical role to maintain the excellence of analytical performances. Implementation of standard operation procedures (SOPs), high-performed instruments, efficient management, well-trained chemists, and actively participating in programs of Round-robin and Proficiency Testing are keys to maintain an outstanding laboratory for chemical analysis.