The quality of upland cotton fiber continues to be improved by breeders, providing longer and stronger cotton fibers capable of producing high-quality spun yarns while withstanding faster processing speeds. An understanding of inheritance of fiber length and strength within and between wide-genetic bases will aid breeders in this effort. Thus, high volume instrumentation (HVI) upper half mean length and HVI fiber bundle strength of five cotton genotypes, representing a wide-genetic base, were subjected to a generation means analysis. Genotypes included one TAM ELS Upland lines, one mutated lines of TAM 94L-25, one experimental line derived through interspecific hybridization exhibiting high length, one experimental upland line exhibiting high strength, and TAMCOT 22 as an average quality check. Parents, their F₁ progeny without reciprocals, F₂, and backcross generations were grown near College Station, TX in 2010 and 2011 in a split-plot design inside a randomized complete block design with four replications. Samples were ginned on laboratory saw gin and sent to Cotton Incorporated for HVI evaluation.  Gene effects and heritability estimates will be presented.