Tylosin, a macrolide class of antibiotics, has been used for disease prevention and growth promotion in swine, beef cattle, and poultry production. This has raised concern over the ecological risks since animal manure is widely applied in agricultural lands as fertilizer. The aim of this work was to investigate tylosin sorption on humic-like fractions extracted from animal waste and poultry waste manure, and humic acid extracted from peat soil as a reference. Dialysis membrane method was employed to conduct this equilibrium binding experiment with several levels of concentration and pH conditions. Prior to this experiment, purified humic-like materials were analyzed their chemicals properties and proton binding characteristics. Tylosin sorption behavior on different humic-like substances was analyzed at several pH conditions. The results of tylosin sorption on different type of humic-like substances were compared with tylosin sorption on humic acid extracted from peat soil. Implications of these results will be discussed.