Poultry manure (broiler litter-BL and composted layer manure-CLM) is a potential source of N for winter wheat production in North Carolina Coastal plain soils. Poultry production is well established in this region resulting in large amounts of waste. Evaluation of the N effect of the source and application strategy on yield and specific yield components strengthens the knowledge for profitable utilization of this organic resource. The objective is to compare poultry waste N source, application time, and rate strategy effect on wheat yield and yield components. Experimental plots were in two research stations, Lower Coastal Plain (Goldsboro loamy sand, Aquic Paleudults) and Tidewater (Portsmouth fine sandy loam, Typic Umbraquults) where BL, CLM, and urea ammonium nitrate (UAN) effect on wheat were compared. Organic inputs were applied in October (incorporated), December/January, and February/March for 2 seasons in a randomized complete block design.  Wheat fertilized with broiler litter and composted layer manure yielded less than when a similar rate of N was applied as UAN in a typical topdress shortly prior to jointing.  Plant available N (PAN) coefficients were calculated by comparing yields from BL and CLM to that of the UAN response curve.  Data collected included tiller density at Feekes’ stage 3.0, wheat yield, head density, seed weight and grain N. The PAN coefficients were higher when poultry manure sources were applied at a rate of 67.2 kg N ha^-1 than 134.4 kg N ha^-1. Plant available N coefficients from poultry waste ranged between 21-61% due to variation in source, environment and application time. Tiller density increased with earlier applications of poultry manures, but this only increased grain yield in cases of suboptimal tiller populations.  The interaction of location, season and N application strategy effect was found to be significant for several parameters.  Grain yield effects were not consistently related to variations in any single yield component (head density, seeds/head, or seed weight).