As a follow-up to our previous study, second year information will be presented addressing questions on haploid induction and doubling, utilizing exotic and elite maize.  These projects result from collaborations between Iowa State Doubled Haploid Facility (http://www.plantbreeding.iastate.edu/DHF/DHF.htm) researchers and USDA-ARS Germplasm Enhancement of Maize (GEM) Project (http://www.public.iastate.edu/~usda-gem/) researchers.  They include 1) doubling success of new elite and exotic populations, and previously identified exotic BC1 populations which failed to double in 2009-2010, 2) a study to identify putative haploid seeds without use of genetic color markers, and 3) utilization of SNP markers as a tool to identify bio-energy traits, and percent of exotic donor contribution among 50 BC1 exotic populations and five temperate elite check inbreds.