Cotton (Gossypium spp.) fibers are known to have fiber quality variability within a plant and across different genotypes. This study was done to evaluate fiber traits such as lint percent and fiber length as given by AFIS (Advanced Fiber Information System) for three genotypes: Acala1517-99, Half and Half and FM832 in College Station, Texas, in 2011. Initial results suggest differences for fiber quality within a plant for all genotypes in this trial.  SSR markers were used to identify polymorphisms among these genotypes to measure the amount of relatedness. Findings from this study will enable cotton breeders to efficiently select breeding lines with enhanced fiber quality.