A High Density Genetic Map for Miscanthus Sinensis That Integrates RAD-Seq and Goldengate SNPs.
Poster Number 116
Monday, November 4, 2013
Tampa Convention Center, East Hall, Third Floor
Siyao Liu1, Lindsay Clark2, Justin Gifford1, John Juvik1 and Erik J. Sacks3, (1)Crop Sciences, University of Illinois, Urbana-Champaign, Urbana, IL (2)University of Illinois, Urbana-Champaign, Urbana, IL (3)University of Illinois-Urbana-Champaign, Urbana, IL
Miscanthus is a bioenergy feedstock crop that has only recently become the subject of modern breeding efforts. It also has more than a 100 year history as an ornamental crop in the U.S., with many cultivars currently sold by the horticulture trade. Miscanthus is a perennial, self-incompatible, C4 grass that is closely related to sugarcane but adapted to temperate environments, making it a good choice for much of the U.S. The efficiency of breeding improved Miscanthus biomass cultivars would be greatly increased by using marker-assisted selection, because phenotypic selection for yield traits must typically be done in the second and third years of field trials. Thus, high-density genetic maps will be critical to Miscanthus improvement. Recently, a framework genetic map for M. sinensis was developed at the Univ. of Illinois based on 658 SNPs using a GoldenGate genotyping array. However, sequencing of restriction site associated DNA tags (RAD-seq) is a promising approach for obtaining thousands of SNPs at lower cost than with GoldenGate. A major goal of the current research was to develop a high-density genetic map of M. sinensis that integrates publicly available RAD-seq and GoldenGate SNPs. The mapping population consisted of 306 F1 progeny of M. sinensis ‘Zebrinus’ x M. sinensis ‘Kaskade’. Segregation of the zebra stripe leaf trait in this population was mapped as an example.