Annually harvested lignocellulosic biomass crop residues require novel storage technologies to optimize industrial use.� Solid state fermentation of biomass feedstock may provide organic chemicals and fibers while reducing the risk of dry-storage procedures.� Corn (Zea mays L.) grain has been used widely to produce ethanol, but little is known regarding appropriate ensilage strategies for stover with high dry matter content.� We tested the effects of D-glucose at 0, 10, 20, 30, and 40 g kg^-1 dry matter (DM) and N (KNO₃) at 0, 5, 10, 15, and 20 g kg^-1 DM in a factorial arrangement on chemical composition and fermentation in corn stover following 1, 7, and 21 days of storage.� Ensilage of 7 days resulted in a pH of 5.2 but a further decline to pH 4.9 at day 21 was not significant.� Lactate rose from 7 g kg^-1 at day 1 to 12.8 g kg^-1 at day 21 but effects of sugar or N levels were inconsistent.� Acetate was similar at day 1 and 7 (average of 5.2 g kg^-1), but increased towards day 21 (6.2 g kg^-1).� Higher sugar levels generally decreased acetate concentrations.� Propionate, butyrate, and isobutyrate averaged during the experiment 0.4 g kg^-1, 0.6 g kg^-1, and 0.7 g kg^-1, respectively.� Neutral detergent fiber increased from 780 g kg^-1 at day 1 to 799 g kg^-1 at day 2 and decreased to 770 g kg^-1 at day 21, but no effects of substrate levels were found during the period of ensiling.� Effects of either sugar or N were also absent in acid detergent fiber, but average concentrations decreased from 480 g kg^-1 at day 7 to 450 g kg^-1 at day 21.� Our results suggested that sugar addition may aid in reducing pH and enhancing preservation but interaction with others substrates warrant further research.�