Broiler litter application based on N needs of cotton ( Gossypium SP) may result in soil accumulation of P and other ions, since broiler litter N/P ratio is usually smaller than the cotton N/P uptake ratio. This study was conducted from 2002 to 2004 on two separate commercial farms representing no-till (Coffeeville, MS) and conventional tillage (Cruger, MS) practices to evaluate the effects of broiler litter application rate with supplementation of inorganic N fertilizer on soil properties. Treatments included broiler litter rates of 2.2, 4.5, and 6.7 Mg ha-1 in combination with 0, 34 and 67 kg N ha-1. The source of commercial N fertilizer was urea-ammonium nitrate solution (UAN). Fertilized ‘farmer standard' and unfertilized checks were also included. Soil surface C and N (0-15 cm ) increased with increasing broiler litter applications and the quantities were greater for No-till than conventional tillage system indicating greater C sequestration with no-till practice in Coffeeville. For both locations, soil surface pH and electrical conductivity (EC) also increased with increasing broiler litter application rate. The quantity of electrical conductivity (EC) was much greater in Cruger (conventional tillage) than Coffeeville (no-till) due to greater soil concentration of K, Ca, Mg at the Cruger site. At both sites, farmer standard treatment decreased both soil pH and EC as compared to broiler litter application. After 3 yr broiler litter applications, residual soil NO3-N was greater in no-till than conventional tillage at the 0-15 cm depth. For both locations, soil bulk density was unaffected by broiler litter applications.