32-2 Use of Molecular Analysis Techniques to Optimize Amphibian Tissues Yield of High Quality and Quantity of DNA.

Poster Number 101

See more from this Division: Students of Agronomy, Soils and Environmental Sciences (SASES)
See more from this Session: Symposium-- National Student Research Symposium Poster Contest
Monday, October 22, 2012
Duke Energy Convention Center, Exhibit Hall AB, Level 1
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Calvin Means, Environmental Science Club, Alabama A & M Environmental Science Club, Normal, AL
Researcher: Calvin Means

Title: Use of molecular analysis techniques to optimize amphibian tissues yield of high quality and quantity of DNA

Summary: Global decline of amphibian populations has been increasing steadily. Traditional methods of analyzing population fitness and disease are limited and can continue for years. Often, the discovery of poor population fitness in amphibians is tragically to late to prevent the occurrence of drastic population decline. The use of molecular techniques to evaluate populations fitness based on variation, have become a preferred method in assessing amphibian declines.  Beebee and Rowe (2001) found genetic analysis using microsatellite loci was effective in distinguishing between short-term fluctuation and long-term trend in accurately assessing amphibian declines. Difficult still exist in extraction DNA from a variety of tissue types. This research project will address the need to determine which, if any, tissue types yield significantly higher quality DNA.

See more from this Division: Students of Agronomy, Soils and Environmental Sciences (SASES)
See more from this Session: Symposium-- National Student Research Symposium Poster Contest