109-14 A High Density Genetic Map for Miscanthus sinensis That Integrates RAD-Seq and Goldengate SNPs.
Poster Number 519
See more from this Division: C01 Crop Breeding & GeneticsSee more from this Session: Crop Breeding and Genetics: II (includes student competition)
Monday, November 3, 2014
Long Beach Convention Center, Exhibit Hall ABC
Miscanthus is a bioenergy feedstock crop that has only recently become the subject of modern breeding efforts. It also has more than a 100 year history as an ornamental crop in the U.S., with many cultivars currently sold by the horticulture trade. Miscanthus is a perennial, self-incompatible, C4 grass that is closely related to sugarcane but adapted to temperate environments, making it a good choice for much of the U.S. The efficiency of breeding improved Miscanthus biomass cultivars would be greatly increased by using marker-assisted selection, because phenotypic selection for yield traits must typically be done in the second and third years of field trials. Thus, high-density genetic maps will be critical to Miscanthus improvement. Recently, a framework genetic map for M. sinensis was developed at the Univ. of Illinois based on 658 SNPs using a GoldenGate genotyping array. However, sequencing of restriction site associated DNA tags (RAD-seq) is a promising approach for obtaining thousands of SNPs at lower cost than with GoldenGate. A major goal of the current research was to develop a high-density genetic map of M. sinensis that integrates publicly available RAD-seq and GoldenGate SNPs. The mapping population consisted of 306 F1 progeny of M. sinensis ‘Zebrinus’ x M. sinensis ‘Kaskade’. Segregation of the zebra stripe leaf trait in this population was mapped as an example.
See more from this Division: C01 Crop Breeding & GeneticsSee more from this Session: Crop Breeding and Genetics: II (includes student competition)