Alkaline stabilized biosolids contain a considerable amount of nitrogen and their land application affect soil N dynamics. We investigated the effects of primary treated (RB) and alkaline-stabilized biosolids (ATB) on corn biomass and labile soil N on Mt.Hope, Lindsay, and Bible Hill soils in, Canada. Soils were amended with ATB at 14, 28 and 42 Mg ha^-1 dry weight, RB at 42 Mg ha^-1 dry weight, inorganic fertilizer (FERT), and control (CONT) with zero N. Corn was seeded into pots in a controlled environment chamber (18-27 ^oC; 16h daylight), and above ground biomass was harvested after 10 weeks. Plant dry matter (DM) was measured and plant tissues were analyzed for N concentration. Soils at harvest were analyzed for N fractions including KCl 2M extracted mineral soil N extracted,  UV absorbance of 0.01 M NaHCO₃ extract at 205 and 260 nm, water extractable N and total N. Mean pH and EC values were 7.3 and 1.1 dS/m in RB, and 10.8 and 10.8 d/S/m in ATB, respectively.  Mean total N and C:N ratio values were 22.3 g/kg and 15.5 in RB, and 15.4 g/kg and 16.4 in ATB, respectively. The dry matter yield and crop N uptake were significantly affected by soil type and fertility treatments. The highest dry biomass and N uptake values were measured in Mt.Hope and for FERT followed by control. Overall, biosolids N did not contribute to plant N uptake. Labile N concentrations were affected by soil type x fertility treatment interactions, while total N was not affected. The Mt.Hope soil with lower C:N ratio and higher silt+clay content interacted differently with biosolids compared with Bible Hill and LRRoad.