308-25 Sequence Comparison of the Starch Branching enzyme1 Gene Among Several Native American Varieties of Maize.

Poster Number 1038

See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: Genomics, Molecular Genetics & Biotechnology: I

Tuesday, November 17, 2015
Minneapolis Convention Center, Exhibit Hall BC

Abiskar Gyawali, SOUTH DAKOTA, South Dakota State University, Brookings, SD, Vivek Shrestha, South Dakota, South Dakota State University, Brookings, SD, Yajun Wu, Biology and Microbiology, South Dakota State University, Brookings, SD and Donald Auger, Dept. of Biology-Microbiology, South Dakota State University, Brookings, SD
Abstract:
Previously, we determined that the allele for starch branching enzyme 1 (sbe1) in a high amylose line, GEMS-0067, would translate into a protein with six amino acid polymorphisms relative to an allele in a lower amylose line, H99ae. A review of the published sbe1 sequence data indicates that four of the amino acid polymorphisms appear to be unique to GEMS-0067, and two are found in grass relatives of maize, but not in other maize varieties that have thus far been characterized. Interestingly, the maize alleles of sbe1 are remarkably uniform, i.e., nearly all have the same amino acid usage as found in H99ae. Most of these maize varieties are inbreds or hybrids and we are curious if the uniformity might have resulted from selection or fixation due to a genetic bottleneck. We are presenting sequence data for sbe1 from a more diverse sample including several teosintes.

See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: Genomics, Molecular Genetics & Biotechnology: I