Comparative Estimation of Influence of a Variable Magnetic Field by Induction of 1500 and 6000 Mktl on Number of Microflora of Chernozem Ordinary, Brown Forest Soil and Grey Forest Soil.
Elena U. Starovoitova, Marina A. Repyah, and Tatyana V. Denisova. Rostov State University, B.Sadovaya str., 105, Rostov-on-Don, 344006, Russia
Studying of influence of electromagnetic fields (EMF) on biological objects - one of actual problems of the present. It is proved, that ability to live, development, evolution of all alive organisms occurred under influence of natural geomagnetic fields and fields of a space origin. Organisms not only adapted to them, but also used them. In the last some decades application of devices which radiate EMF, has considerably increased. The total of the industrial, scientific and medical installations radiating EMF, in the world is estimated in some hundreds millions, and their number constantly increases for 3-7% one year. It is one of kinds of power environmental contamination and soil, in particular. A plenty of researches is devoted to studying of influence EMF on pure cultures of microorganisms. Oppressing influence of MT. Absence of influence of MT on microorganisms marked. While separate works are devoted to influence EMF on soil microflora. The microorganisms living in ground not only its inhabitants, but also the organisms playing the leading part during soil formation. The basic representatives of soil microflora are bacteria, actynomycetes, microscopic seaweed and micromycetes. Studying influence EMF on biological properties of soil, in particular soil microflora therefore is obviously important. In this connection the purpose of research was studying influence of variable magnetic field (VMP) by an induction 1500 and 6000 mkTl on number of microflora of chernozem ordinary, brown forest soil and grey forest soil. Objects of research were the following soils: chernozem ordinary, brown forest soil and grey forest soil. The soil samples of chernozem ordinary were taken from the plow layer (0-30 cm) in May 2003 at the training farm “Donskoi” (Oktyabr'skii district, Rostov region). Samples of brown forest soil were taken from the plow layer (0-30 cm) in May 2004 (Republic of Adygea). Samples of grey forest soil were taken in May 2004 in Kamensk area of the Rostov region. Air-dried soil samples put into glass vessels, humidified water (60% FH) and placed into the solenoid for 5 days. Dozes of EMF were taken by induction of 1500 and 6000 mkTl (industrial frequency of 50 Hz). After the ending of an exposition in skilled and control samples determined the number of soil microorganisms: ammonifying bacteria on MPA, micromycetes on Czapek's by the standard techniques. Experience put in 3 multiple frequencies. Values in control samples accepted for 100%. Results of research have shown, that on number of microflora of brown forest soil VMP did not render influence - authentic differences from the control is not marked. At bacteria some increase in number, but authentically not distinguished from control values is observed. On number of microflora of chernozem ordinary and grey forest soil magnetic fields of the investigated dozes rendered overwhelming influence, but various sensitivity influence of MP of bacteria and microscopic mushrooms is marked. Bacteria are more sensitive than micromycetes. Number of ammonifying bacteria of chernozem ordinary is lower than the control over 58% (p<0,05) and 32% (p<0,05) under influence VMP by an induction of 1500 and 6000 mkTl accordingly, on a bacterium grey forest soil VMP rendered the even greater overwhelming influence - their number is suppressed on 76% (p<0,01) and 88% (p<0,01) accordingly. At micromycetes of chernozem ordinary and grey forest soil the return tendency is observed: micromycetes of grey forest soil are steady against influence VMP - insignificant decrease in their number in comparison with the control not authentically, and number of micromycetes of chernozem ordinary is suppressed on 26% (p<0,01) and 15% (p<0,01) under influence VMP by an induction of 1500 and 6000 mkTl accordingly. Investigated soils on sensitivity of bacteria to VMP by induction of 1500 and 6000 mkTl it is possible to present to a line: grey forest soil>chernozem ordinary> brown forest soil. Sensitivity of micromycetes of investigated soils to VMP - the following line: chernozem ordinary> grey forest soil > brown forest soil. Thus, microflora of brown forest soil and micromycetes of grey forest soil are steady against influence VMP by induction of 1500 and 6000 mkTl. Bacteria of chernozem ordinary and grey forest soil are more sensitive to influence VMP, than micromycetes of these soils.