Sulphur and phosphorous are nutrients known to improve both the quantity and quality of the produce. Sulphur has a profound influence on protein synthesis. Thus, legumes have remarkable affinity for P and S. Pulse crops fix atmospheric nitrogen and require only small amount through fertilizers, however, they require as much as P and S even more than cereal crops. Phosphorous and sulphur application together appears to have synergistic or antagonistic effects. Furthermore, sustainable crop production needs judicious use of fertilizers along with biofertilizers. Keeping this in view the present study was under taken. A field experiment was conducted on a sandy loam soil having pH 8.4; EC 0.79 d Sm^-1; CEC 8.7 cmol (p+) kg^-1; organic carbon 3.1 g kg^-1; available N 180 kg ha^-1 P 18 kg ha^-1, K 280 kg ha^-1 and S 8.2 mg kg^-1 soil. The experiment was conducted in factorial randomized block design with three replications. The treatments included two levels of Rhizobium inoculation of seeds I0 (without inoculation) and I1 (with inoculation); three levels of S ( 0, 20, 40 kg S ha^-1) and three levels of P ( 0, 30, 60 kg P₂O₅ ha^-1). Phosphorus and sulphur were applied as basal doses through DAP and elemental sulphur, respectively. A uniform dose of N was applied with all the treatments. Effect of treatments was studied in terms of plant growth, nodulation, grain and straw yield, nutrient uptake and protein content. Black gram responded significantly to the application of phosphorous, sulphur and Rhizobium inoculation. Rhizobium inoculation of seeds application of sulphur at 40 kg ha^-1 and phosphorous at 60 kg ha^-1 produced 1068.8, 1045.0 and 1058.3 kg ha^-1 grain yield, respectively. Maximum grain yield (1098.2) was recorded with Rhizobium inoculation, application of sulphur at 40 kg ha^-1 and phosphorous at 30 kg ha^-1.These treatments significantly increased the uptake of N, P, K, S in comparison to control. Protein content in grains increased with application of S and P individually but decreased when they were applied in combination at higher doses. Sulphur application along with Rhizobium inoculation also increased the protein content in grains. Keywords: Sulphur, Phosphorous, Rhizobium.