The sub-basin of Motatan river is one of the sectors of bigger agro-social-economic importance of Merida and Trujillo states given the high levels of production of vegetables of high lands. At the moment, it exhibits a high level of deterioration of their natural resources, requiring recuperative measures through plans of management conservationist of soils and waters, with the purpose of continuing maintaining the productivity of the lands and that guarantees their agroecological sustainability. The general objective of this work was to determine the incidence of 9 causes and 7 effects on the agroecological and environmental deterioration in 4 samples areas located in La Venta, Miranda municipality, Merida state, Venezuela. The established approach for the selection of the study areas was based on the more important way of organization that the inhabitants have in that municipality, the Irrigation Committees. Taking into account the levels of reference obtained starting from some preliminary visits the Irrigation Committees “Cruz Chiquita”, “Alisal-El Pedregal”, “El Rincon de La Venta” and “El Rincon del Picacho” was selected with the purpose of carrying out the proposed analysis. The basic premise that guides the purpose of this work resides in a holistic vision of the hidrographic basin concept like an unit of territorial planning for the rational use of the natural resources by a particular population, taking into account physical, economic, environmental, social, political, cultural, educational and financial components, among others; with the purpose of analyzing the degradetive factors and processes of the ambient, looking for the maintenance or the improvement of the lands productivity, necessary for the achievement of the agroecological sustainability of the hidrographics basins. The methodological procedure includes 7 stages: Stage 1: Revision of basic information; Stage 2: Preparation of the project; Stage 3: Preliminary visit to the study area; Stage 4: Selection of the areas study object; Stage 5: Characterization of the areas study object; Stage 6: Application of the Cause–Effect matrixes and Stage 7: Global analysis of the agroecological and environmental deterioration. In accordance to the obtained results is evident that the High Motatan subbasin exhibits, globally, a condition of strong agroecological and environmental, deterioration. The Global Deterioration Index (GDI) for the 4 Irrigation Committees are over 50%. Indeed, the GDI corresponding to the committees “Cruz Chiquita", “Alisal-El Pedregal", “El Rincon de La Venta” and “El Rincon del Picacho” are 62%, 66%, 68% and 57%, respectively. That global deterioration is caused, mainly, for the lack of technical management and of financial resources directed to the solution of the agricultural and environmental problems of the subbasin under study. The more evident consequence associated to these two causes is the decrease of the quality life of the inhabitants of the place. Its concludes that the analysis procedure used to determine the agroecological and environmental deterioration in a sector of the High Motatan Subbasin, starting from the cause-effect analysis, is a valid and important method to know the group of factors and natural processes and antropics that are more associated with the agroecological and environmental deterioration of an ecosystem, its impacts and the specific correctives in terms of practical and uses of the land, guided to potentialize the sustainability of the subbasin with the purpose of production of water for ecological, agricultural, human and agroindustrial uses. Key words: Cause-effect matrix, hidrographic basin, agroecological and environmental deterioration.