Biological activity has important role on chemical behavior of trace elements in soils. In order to study the effect of elimination of microorganisms on trace elements uptake in maize (Zea mays), a greenhouse experiment was conducted with two different soils (calcareous and non-calcareous) and three different treatment on soils (sterile, re-inoculate and non sterile) in a factorial complete randomized design with three replications. Maize irrigated with distilled water for four months. In both soils, sterilization increased concentrations of Mn, Fe and Cu in plant, but that of Zn remained unchanged. The increase of Fe and Cu in plant was higher in non-calcareous soil, whereas plant Mn content was greater in calcareous soil. Re-inoculated autoclaved soil increased concentrations of Mn of plant in both soils. Concentration of plant Fe in calcareous soil and that of plant Cu in non-calcareous soil increased with re-inoculation, however, plant Zn remained unchanged in both soils examined. Despite sterilization caused release of Fe, Cu, and Mn into the soil, the yield of maize in sterile and re-inoculated soil decreased. Sterilization and re-inoculation did not alter soil pH, EC, and organic carbon significantly in the soils tested. In general, elimination of microorganisms in soil by sterilization influenced plant yield and trace elements uptake by maize. Keywords: microorganism, sterilization, soil re-inoculation, trace elements, maize