Saturday, 15 July 2006
141-7

Screening of Natural Multi-functional Bacterium Strain.

Ruzhen Jiao Sr., Research Institute of Forestry, Chinese Academy of Forestry, Wan Shou Shan, Beijing, 100091, China

The paper deals with the process of selecting the strains of multi-functional bacteria. In consideration of the impact on the environment by the release of genetic engineering strains, the screening of natural multi-functional bacterium strains is of particular importance. By inoculating the medium with Ca3 (PO4) 2 and lecithin as the only P source respectively with the strains of bacterial fertilizer in the laboratory, 9 strains of phosphorus-dissolving bacteria were selected. Then a test of the invert intensity was made. In comparison with the controls, the contents of available phosphorus increased from 33.122μg.ml-1 to 343.86μg.ml-1 in the case of the medium with Ca3 (PO4) 2 as the only P source.While the contents of available phosphorus increased from 0.720 to 42.973μg.ml-1 in the case of the medium with organic P lecithin as the only P source. The 9 strains screened out went through an antagonistic test with 3 strains of pathogenic microbes (Erwinia sp.,Pseudomonas sp.,Xanthomonas sp.), 2 multi-functional strains of Micrococcus sp. and Pseudomonas fluorescens Migula KO were selected that could dissolve phosphorus, restrain bacterium and increase seedling growth. Key Words: Bacterial manure, Inorganic phosphorus, Organic phosphorus, Phosphorus-dissolving bacteria, Available phosphate, P-release, Antagonistic effect, Multi-functional strain


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