Saturday, 15 July 2006

Advanced Screening Method for the Selection of Salt Tolerant Crops Using Agar Plate.

Sei Joon Park1, Myoung Yong Shim1, Ju-Young Lee2, Sang Eun Lee2, Su-Yeon Lee2, Sung Yung Yoo2, and Tae Wan Kim3. (1) Institute of Ecological Phytochemistry, Hankyong National Univ, 67 Seonjung-dong, Anseong, 456-749, South Korea, (2) Division of Plant Nutrition, National Institute of Agricultural Science and Technology, 250, Seodundong, Kwonseongoo, Suwon, South Korea, (3) Department of Plant Resources Science, Hankyong National Univ, 67 Seogjung-dong, Anseong, 456-749, South Korea

Seeds of sugar beet, barley, maize, lettuce and rape were rinsed in deionized water and then soaked in 5% NaOCl for disinfection. For germination test, 3 to 7 seeds were immersed on 1% agar plate with 50 mM NaCl and pH indicators. Bromocresolpurple with 1 mM NH4Cl and bromocresolblue with 1 mM NaNO3 were used to detect the pH changes during the germination on agar plate in the range from 6 to 4 or from 4 to 6, respectively. The germination of monocots was rapidly progressed comparing to dicots. It was clearly observed that sugar beet, barley and rape were tolerant to salt stress at least during the germination stage. Similarly, young seedlings were immersed into 1 % agar medium with 50 mM NaCl and pH indicators. Rhizospheric pH changes were to be observed for 72 hours. The pH changes near root were detected within 2 hours. We have tried to identify precisely rhizospheric pH changes with some other pH indicators. Colors were transformed to numeric values on Photoshop program using digital camera and then statistically evaluated. Numeric values were closely correlated with pH values. Thus, we would conclude that germination and young seedling tests on agar plate with pH indicators can rapidly detect the rhizospheric stress. Key words: germination test, rhizospheric pH, screening, salt stress. Acknowledgment: This study was financially supported from Bio-Green 21 project, RDA and in part from GRRC project (No. 3-1), Hankyong National University. Figure. 1. The changes of rhizospheric pH in barley seedling 36 hours after immersing into 1 % agar plate with pH indicators.

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