We tested the effect of two organic amendments (straw and resinous compost compared to a non-amended situation) on the soil copper impact using biological and chemical descriptors. A microcosm study was designed during 35 days in the absence or presence of copper, and CO2 measurements, biomass, number of total cultivated and of copper resistant bacteria were analysed, as well as water-extractable total organic carbon and copper, pH and the distribution of soluble organic carbon in small (<500 Da) and high molecules after ultrafiltration. Biological descriptors indicate a copper effect as soon as 15 days for the non-amended sample, only after 35 days in the case of straw inputs, while no copper effect is observed for the resinous compost sample. Chemical descriptors show the same trends, and a relation can be established with some biological descriptors, allowing to better understand the dynamic of copper and carbon in these systems. The results stressed the importance of the nature of the organic amendments on the soil biological and chemical response after copper addition, and the importance of TOC dynamic as a chemical descriptor of the impact