2008 Joint Annual Meeting (5-9 Oct. 2008): Characterization of Manure from Conventional and Phytase Transgenic Pigs by Advanced Solid-State NMR Spectroscopy.
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607-21 Characterization of Manure from Conventional and Phytase Transgenic Pigs by Advanced Solid-State NMR Spectroscopy.



Monday, 6 October 2008
George R. Brown Convention Center, Exhibit Hall E
Jingdong Mao1, AYODELE AJAKAIYE2, Yeqing LAN1, Daniel Olk3, Michael CEBALLOS4, Tiequan Zhang5, MING Z. FAN2 and CECIL W. FORSBERG2, (1)Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA 23529
(2)Molecular and Cellular Biology, University of Guelph, N1G 2W1, Guelph, ON N1G 2W1, Canada
(3)USDA-ARS National Soil Tilth Lab., 2110 University Boulevard, Ames, IA 50011-3120
(4)Salish Kootenai College, Pablo, MT 59865
(5)Agriculture and Agri-Food Canada, 2585 County Road 20, Harrow, ON N0R 1G0, CANADA
Non-point phosphorus (P) pollution from animal manure is becoming a serious global problem. The current solution for the swine industry is including the enzyme phytase as a component of the cereal grain diet. A very real possibility in the future is the production of transgenic pigs that express phytase in the salivary glands and secrete it in the saliva. This study provides a detailed chemical structure of manure from conventional pigs and transgenic pigs that express phytase using new solid-state NMR techniques. Spectral editing techniques and quantitative NMR techniques were used to identify and quantify specific functional groups. Two-dimentional 1H-13C heteronuclear correlation NMR was used to detect their connectivity. Manure from conventional and transgenic pigs had similar peptide, carbohydrate, and fatty acid components, while those from transgenic pigs contained more carbohydrates and fewer nonpolar alkyls. There was no consistent effect from diet with or without supplemental phosphate or growth stage.
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