2008 Joint Annual Meeting (5-9 Oct. 2008): Identification of SNP Markers Associated with Oleic Acid QTLs in Soybean.
Start | Author Index

656-2 Identification of SNP Markers Associated with Oleic Acid QTLs in Soybean.



Tuesday, 7 October 2008
George R. Brown Convention Center, Exhibit Hall E
Maria J. Monteros, Forage Improvement Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, Bo-Keun Ha, Center for Applied Genetic Technologies, Agtech, 111 Riverbend Road, Athens, GA 30602 and H. Roger Boerma, University of Georgia, Center for Applied Genetic Technologies, 111 Riverbend Road, Athens, GA 30602
Increasing the amount of oleic acid in soybean oil would reduce the need for hydrogenation, a process that creates unhealthy trans fatty acids.  A previous study mapped oleic acid QTLs from N00-3350 (~550 g kg-1 oleic acid) soybean to linkage groups (LG) A1, D2, G, L, and O.  The objectives of this study were to develop high throughput assays to detect single nucleotide polymorphism (SNP) markers from fatty acid pathway genes and sequence-tagged sites in regions of the soybean genome associated with oleic acid QTLs.   A F2:3 population consisting of 188 individuals from the cross of a glyphosate-resistant backross conversion of Boggs, G99-G725, (~206 g kg-1 oleic acid) x N00-3350 was screened using melting curve analysis of fluorescent labeled SimpleProbes.  A set of 32 genotypes which represent 95% of the genes in the genetic base of North American soybean were also screened.  SNPs from fatty acid candidate genes have been identified on LG A1, D2, G, and O.  The assays developed for detecting SNP markers associated with oleic acid QTLs are based on melting curve analysis.  These markers provide a high-throughput and rapid SNP genotyping method which can be used to accelerate the development of mid-oleic acid content soybean cultivars.
See more of: Genetics of Nutrition and Quality (Posters)
See more of: C07 Genomics, Molecular Genetics & Biotechnology