2008 Joint Annual Meeting (5-9 Oct. 2008): Swine Lagoon Effluent as a Potential Source of Antibiotic Resistant Genes in Surface Water.
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668-1 Swine Lagoon Effluent as a Potential Source of Antibiotic Resistant Genes in Surface Water.



Tuesday, 7 October 2008
George R. Brown Convention Center, Exhibit Hall E
Lloyd Liwimbi1, Alexandria Graves2, Daniel Israel3, Bradford Robinson2, Joie Lubbers2, Daniel Vaal2 and Charles Cahoon2, (1)Soil Science, North Carolina State University, 15006 BRAGAW HALL, 210 DAN ALLEN DR, Raleigh, NC 27606
(2)Soil Science, North Carolina State University, Campus Box 7619, Raleigh, NC 27695-7619
(3)3131 Williams Hall, Box 7619, USDA-ARS, North Carolina State University, Soil Science Dept. USDA-ARS, Raleigh, NC 27695
The use of antibiotics in animals is suspected to be a major route of the transference of antibiotic resistant bacteria to humans, even when different antibiotics are used in animals than in people.   North Carolina is the home of our Nation’s second largest swine industry.  Most of this swine production is restricted to a small geographical area in southeastern North Carolina. This high concentration of swine production may increase the risk of antibiotic resistant bacteria from swine operations reaching the nearby surface waters.   The goal of this study is to identify and quantify E. coli isolates with  antibiotic resistant genes in raw swine manure, lagoon effluent from a commercial swine facility and in nearby  ground and surface waters. To date, 900 E. coli strains from swine manure, lagoon effluent and nearby ground and surface waters have been recovered for the purpose of evaluating for antibiotic resistance genes and the phenotypic expression of the resistance.  E. coli isolates have been evaluated for the phenotypic expression of antibiotic resistance to various concentrations of the following antibiotics: erythromycin, neomycin, oxytetracycline, streptomycin, tetracycline, cephalothin, apramycin, sulfamethoxazole, and trimethoprim-sulfamethoxazole.  One hundred percent of the E. coli isolates displayed multiple antibiotic resistances.  Genotypic evaluation for antibiotic resistance genes (aadA, strA, strB, tetA, tetB, tetC, sul1, sul2, sul3, and aac(3)IV) in the E. coli isolates is underway.  The distribution of antibiotic resistant genes in swine-manure derived bacteria in shallow groundwater near the stream or in the stream would document the need for improved mitigation strategies.
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