102-5 Correlating Attachment Behavior with Cell Properties for Eight Porcine Escherichia Coli Isolates.

See more from this Division: S03 Soil Biology & Biochemistry
See more from this Session: Microbial Responses to the Environment: I
Monday, November 1, 2010: 2:20 PM
Long Beach Convention Center, Room 104B, First Floor
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Carl Bolster1, Kimberly Cook1, Ian Marcus2, Berat Haznedaroglu2 and Sharon Walker2, (1)USDA-ARS, Bowling Green, KY
(2)University of California, Riverside, Riverside, CA
In this study we investigate how growth stage and depositional environment affect variability of cell properties and transport behavior of eight porcine E. coli isolates. We compared the surface properties for cells harvested during exponential and stationary growth phase and their transport behavior through columns packed with either uncoated or Fe-coated quartz sand. We then investigated correlations between measured cell properties and fitted bacterial attachment efficiencies. For both growth stages we found that bacterial attachment efficiencies to the uncoated quartz sand varied among the eight different isolates by over an order of magnitude whereas attachment efficiencies to the Fe-coated sands varied by a factor of less than two. With the exception of one isolate, growth condition had minimal impact on attachment efficiencies to the uncoated sands. A strong and statistically significant inverse relationship was observed between bacterial attachment to uncoated quartz sand and log-transformed zeta potential whereas a mild yet statistically significant relationship between bacterial attachment to the Fe-coated soils and cell width was observed. For the experimental conditions used in our study, we found that variability in E. coli transport was more dependent on the depositional environment than on growth conditions.
See more from this Division: S03 Soil Biology & Biochemistry
See more from this Session: Microbial Responses to the Environment: I