Identification of Organic Phosphorus Forms In Forest Soil Using 31p Nuclear Magnetic Resonance Spectroscopy.

  A phosphorus fractionation method and ³¹P Nuclear Magnetic Resonance (³¹PNMR) spectroscopy was utilized to identify the transformation of organic phosphorus (P) forms in a forest soil subjected to thinning and burning treatments. Iron bound P was extracted using bicarbonate/dithionite solution.  Organic P and polyphosphates bound to Al was extracted using 0.1M sodium hydroxide. Humic acid bound P was separated by acidification of the sodium hydroxide extract. ³¹P NMR studies indicate the dominant organic P forms in the extracts are monoester-P followed by diester-P. Variations were observed in ratios of phosphate monoester and diester-P in soils subjected to logging and burning treatments.