Assessments of Genetic Relationships, Diversity and Genome Structure of Some Commen Vetch (Vicia sativa L.) Lines.

The objectives of this study were to estimate diversity and genetic similarity distribution and to reveal nuclear DNA contents (C-value) of 30 accessions of Vicia sativa L. that represented the genetic base of natural flora, national and international germplasm resources. Fifteen sequence-related amplified polymorphism (SRAP) and 20 sequence tagged microsatellite site (STMS) primers were used to amplify genomic profiles.  Based on Nei’s standard genetic distances, both cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCoA) separated two main clusters. This study revealed no significant C- value differences among accessions although useful polymorphisms among diverse accessions were present and PCR base markers allowed their genetic relationships to be efficiently deduced.

 Key words: Vetch, Vicia, SRAP, STMS, C-value, phylogenetics