220-14 Characterization of the Genetic Diversity of Perennial Grasses Using Genotyping by Sequencing.

See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Crop Breeding and Genetics: Maize and Perennial Grasses
Tuesday, October 18, 2011: 4:35 PM
Henry Gonzalez Convention Center, Room 206B
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Fei Lu1, Alexander E. Lipka2, Robert Elshire1, J.C. Glaubitz1, Jerome H. Cherney3, Michael Casler4, Edward S. Buckler5 and Denise Costich6, (1)Cornell University, Ithaca, NY
(2)Plant Breeding Soil Nuitrition, USDA-ARS, Ithaca, NY
(3)Department of Crop and Soil Sciences, Cornell University, Ithaca, NY
(4)USDA-ARS, Madison, WI
(5)Plant Breeding and Genetics, Cornell University, USDA-ARS, Ithaca, NY
(6)USDA-ARS, Robert Holley Center, Institute for Genomic Diversity, Cornell University, Ithaca, NY
Switchgrass (Panicum virgatum L.)  and reed canarygrass (Phalaris arundinacea L.) are perennial grasses which have high biomass production potential. To enable genomic selection and genome wide association analysis of these species, germplasm resources, including both bi-parental linkage populations and association populations, have been developed. Unlike traditional genotyping methods, genotyping by sequencing (GBS) is a high-throughput, low-cost way to explore the genetic diversity in populations. About 2000 individual plants are being genotyped using GBS. As many as 100,000 high density SNP markers have been generated. These markers will provide unparalleled insights into genomic variation, polyploidy, diversity and population structure. This same germplasm has been simultaneously phenotyped for multiple morphological and developmental traits, allowing genomic selection and genome wide association study to be brought into practice.
See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Crop Breeding and Genetics: Maize and Perennial Grasses