123-16 Agricultural Practices Affecting Soil Carbon Sequestration Cause Shifts In Microbial Community Assembly As Revealed by Tag Encoded Pyrosequencing.



Monday, October 17, 2011
Henry Gonzalez Convention Center, Hall C, Street Level

Suhana Chattopadhyay1, Michelle Haddix2, Richard Conant2, Sherri Morris3, Eldor Paul2 and Christopher Blackwood1, (1)Department of Biological Sciences, Kent State University, Kent, OH
(2)Natural Resource Ecology Laboratory, Colorado State University, Fort Collins, CO
(3)Bradley University, Peoria, IL
Management induced microbial community shifts may be linked to soil organic matter stability. The objective of our study is to elucidate how microbial composition shifts with different agricultural practices. Soil samples (0-20 cm depth) were collected from Hoytville, Ohio.  Experimental treatments included native forest (NF), conventional tillage maize (CT) and no tillage maize (NT). Partial ribosomal amplification on extracted DNA with tag encoded primers and 454 FLX titanium pyrosequencing were used to estimate microbial diversity and community composition. After quality trimming, 62639 bacterial and 413,940 fungal sequences were clustered at 97% similarity into 7171 and 4605 OTUs, respectively. Rarefaction curves of OTU diversity kept increasing for bacteria at 2400 OTUs but leveled off at ~600 OTUs for fungi. There were no significant differences in species diversity (Simpson and Shannon indices) and OTU richness among treatments. Relative abundance of Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes was higher in disturbed systems than forest, in contrast to Acidobacteria and Verrucomicrobia. Redundancy analysis showed 65% of the variation in bacterial communities (p=0.015) and 40% of variation in fungal communities (p=0.01) was explained by treatments. This study demonstrated that the soil microbial community structure shifts with implementation of different agricultural practices known to influence the amount of carbon stored in soil. Further studies are determining carbon pool size and flux on these sites and how treatments affect microbial community composition at different stages of decomposition over long periods of time.
See more from this Division: S03 Soil Biology & Biochemistry
See more from this Session: Microbe, Plant , and Soil Interactions (Includes Graduate Student Poster Competition)