320-10 Chromosome Doubling of Haploid Maize Seedlings by Various In Vitro Treatments.



Wednesday, October 19, 2011
Henry Gonzalez Convention Center, Hall C, Street Level

Adam Vanous1, Andrew Smelser2, Michael Blanco2, Thomas Lubberstedt1, Ursula Frei1 and Candice Gardner2, (1)Department of Agronomy, Iowa State University, Ames, IA
(2)USDA-ARS-PIRU, Ames, IA
A two-year study was conducted on methods for doubling haploid maize plants with elite temperate backgrounds following various seed treatments.  Seedlings were treated with colchicine or other chemicals by 1) injecting plants and 2) a soak method using various seedling treatments.  Treated seedlings were transplanted in the field in 2010 and 2011, and doubled plants that shed pollen and produced ear shoots were pollinated to derive double haploid inbred lines.  Results and conclusions will be reported for efficacy of doubling, and other morphological traits observed in the field.
See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Molecular, Statistical and Breeding Tools to Improve Selection Efficiency