109-10 Ongoing Results From a Candidate Gene Pipeline for Testing the Effect of DNA Sequences On Aflatoxin Accumulation and A. Flavus Resistance in Maize.

Poster Number 735

See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: Genomics, Molecular Biology, and Biotechnology Advances for Crop Improvement
Monday, October 22, 2012
Duke Energy Convention Center, Exhibit Hall AB, Level 1
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Marilyn Warburton1, W. Paul Williams2, Leigh Hawkins1, Erik Mylroie1, Seval Ozcan3, Xueyan Shan3, Prem Choury4, Eric Schmelz4, Alisa Huffaker4, Seth Murray5, Michael Kolomiets5, Gerald De La Fuente5, Baozhu Guo6 and Dafne Alves7, (1)USDA-ARS CHPRRU, Mississippi State, MS
(2)USDA-ARS, Mississippi State, MS
(3)Mississippi State University, Mississippi State, MS
(4)Chemistry Research Unit, USDA-ARS Chemistry Research Unit, Gainesville, FL
(5)Soil and Crop Sciences, Texas A&M University, College Station, TX
(6)Crop Protection and Management Research Unit, USDA-ARS, Tifton, GA
(7)Universidade Federal de Mato Grosso, Cuiabá, Brazil
Poster Presentation
  • CandGenePipePost.docx.pdf (560.3 kB)
  • Many projects have identified candidate genes for resistance to aflatoxin accumulation or Aspergillus flavus resistance in maize using genetic mapping, large scale expression, or proteomics studies. However, only a small percentage of these candidates have been validated under field conditions and their relative contribution to resistance, if any, is unknown.   Here, we present the ongoing results of a candidate gene testing pipeline that consists of steps for identifying, testing, and verifying the association of any maize gene sequence with resistance.  The pipeline includes four QTL mapping populations and one association mapping panel, all of which have been fully phenotyped over multiple years, locations, and replications for aflatoxin accumulation resistance and associated phenotypes.  These populations have also all been genotyped with low density markers in the case of the QTL mapping populations, and high density SNP markers in the case of the association panel.  More than two dozen genes identified as possible resistance candidates from a database of previous studies, current ongoing studies, or published results, have been put into the candidate gene testing pipeline. SNPs and InDel polymorphisms within each gene that map to the correct genomic location are tested for phenotypic effect on aflatoxin accumulation resistance, and results are presented here.
    See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
    See more from this Session: Genomics, Molecular Biology, and Biotechnology Advances for Crop Improvement