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Genetic Diversity Among Soybean Genotypes Selected for Hybridizations and Directed for Rotation with Sugar Cane.
Poster Number 408
Tuesday, October 23, 2012
Duke Energy Convention Center, Exhibit Hall AB, Level 1, 1
Daniel Leite1, Mariana Pazeto1, Eduardo Bizari1, Sandra Unêda-Trevisoli2 and Antonio Di Mauro1, (1)UNESP - São Paulo State University, Jaboticabal, Brazil (2)UNESP - São Paulo State University, Jaboticabal, Brazil
Soybean has assumedincreasing importance insugarcane areasof São Paulo State(Brazil) as an option for the cultivationof rotation with sugar cane.However, it is necessarythat thecultivationhasa short life cycletomeetthescheduleof sugar cane,as well as goodagronomic traits. Thus, we seek to obtainpromising soybean genotypes forsugarcane rotation systemthroughartificial crossingsbetweenparentalcarriesearliness, yield grain, disease resistance andhigh oil content. Within this focusthe objective of thisstudy was to evaluate the promising parental with genetic diversity for these characters, allowing the identification of the most contrasting parental for artificialhybridization, resulting in greatergenetic variability. We used 57 Simple Sequence Repeat (SSR) markers to assess the genetic diversity of 23 soybean genotypes. The SSR markers were assessed using 3% (w/v) agarose gels stained with ethidium bromide and genotyped considering the presence or absence of bands. The results of molecular analyzes provides a standard of 135 polymorphic bands that were subjected to clustering analysis using Jaccard similarity coefficients and the Unweighted Pair Group Method with Arithmetic mean (UPGMA). The constructed dendrogrampresenteda maximum Jaccard indexof 0.66, indicating a moderategenetic diversityamong the evaluated genotypes. However, there was formationof 13distinct groups, being L1 and Orba, the most genotypes divergent. Through this analysis will be possible to direct the crossings to obtain a greater genetic variability between the selected promising parental for the desired attributes.
