Soybean oil is composed of five different fatty acids, stearic, oleic, palmitic, linolenic, and linoleic, and is important for cooking and industrial purposes. Elevated amounts of stearic acid in soybean oil may be useful in some baking fats and other uses. Stearoyl-acyl carrier protein desaturase (SACPD) is a major gene involved in the biosynthesis of stearic acid in the seed. The soybean genome contains three genes encoding SACPD. N-nitroso-N-methylurea (NMU) mutagenized lines were screened for elevated stearic acid levels.  Wild type soybeans contain 3-5% stearic acid and stearic acid content in the selected mutants ranged from 7-15%. We investigated whether the high stearic acid lines contained mutations in the SACPD gene. Tissue samples were collected and the DNA was extracted to amplify SACPDA, SACPDB, and SACPDC. This revealed a mutation (G2201T) in exon 2 of SACPDC in one candidate line, but no mutations on SACPDA, SACPDB, or any other region of SACPDC. The mutation resulted in an amino acid change from arginine to serine. Other lines contained no mutation in SACPDA, B, or C. The negative results provide valuable data suggesting that additional genes that contribute to high stearic acid levels lie elsewhere in the genome.