Perfluorooctanoic acid (PFOA), a representative of perfluorinated chemicals (PFCs), is globally distributed in terrestrial and aquatic ecosystems. Understanding PFOAs transformation in soil is a key in predicting and managing this persistent organic pollutant. Our present study revealed that transformation of PFOA in soil can be enhanced through reactions mediated by laccase. Experiments were conducted by incubating a Cecil sandy clay loam soil preloaded with 10 µg PFOA g^-1 soil. At 45 days of incubation with laccase application (2 U 48 hours^-1 g^-1 soil) extractable PFOA reduced by 42.9%. We examined the effect of three chemicals, namely violuric acid (VA), 1-hydroxybenzotriazole (HBT), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), which acts as laccase mediators.  The extractable PFOA was 1.6% for laccase-HBT, 5.3% for laccase-ABTS, and 9.9% for laccase-VA systems after 45 days of incubation period. Effect of natural organic matter (5 µg g^-1 soil) was also determined on extractable PFOA in laccase and laccase-mediator system. The results indicate that enzymatic humification reactions may comprise an important natural transformation pathway for PFOA in soil, and that appropriately engineered enzymatic processes may hold a potential for treatment of PFOA contaminated soil and aquatic systems.