In Vitro Propagation of Native Bigtooth Maple (Acer grandidentatum).

Tissue culture has not been used to propagate mature tissue of native bigtooth maple (Acer grandidentatum).  The purpose of this experiment was to develop a protocol to root hardwood cuttings by tissue culture.  Hardwood cuttings were acquired from nursery grown trees at Utah State University. The cuttings were divided into four treatments: 10 µM indole-3- acetic acid (IAA), 10 µM l –naphthaleneacetic acid (NAA), 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and 10 µM indole-3-butyric acid (IBA). Each cutting was randomly chosen from different sections of the stem. Furthermore each cutting contained one and/or two nodes per explant. Shoot proliferation occurred with the explants exposed to IAA, NAA, and 2,4-D. After a period of 27 days, several successfully budded explants were moved to a non-growth hormone media for later comparison with those cuttings which remained in growth hormone.