Comparative Analysis of Soybean Accessions Using ISSR and RAPD Markers.

Soybean (Glycine max) offers potential to increase the genetic variability and to provide desirable characteristics for soybean improvement. Diversities in many crops such as soybeans have been documented based on morphological and agronomical traits. Such characters are strongly influenced by environmental factors and the developmental stage of the plant. The main objectives of the present study were 1) to determine differences between ISSR (Inter Simple Sequence repeats) and RAPD (Randomly Amplified Polymorphic DNA) marker systems in detecting genetic variation in soybeans and corn and 2) to identify and characterize accession- specific molecular markers in soybean. Genomic DNA from soybean (Glycine max) accessions from different provenances was analyzed using polymerase chain reaction (PCR). These DNA samples were amplified with several ISSR and RAPD primers. We hypothesis that ISSR primers will generate more genetic variation than RAPD primer and that soybean lines from different countries can be differentiated using molecular markers. Accession specific ISSR, RAPD markers will be identified; cloned and sequenced they will be converted to SCAR markers. These markers can be used for molecular breeding of soybean.