Comparison Of Three Different Cytoplasms In The Ogura Inra Cytoplasmic Male Sterility Pollination Control System.

The Ogura INRA cytoplasmic male sterility (CMS) system is the dominant pollination control system for the development of canola/rapeseed (Brassica napus) hybrids in North America. The ogu-INRA CMS pollination control system is derived from the protoplast fusion between Raphanus sativus and B. napus. These cybrids contain the R. sativus mitochondrial gene orf138 and retain B. napus chloroplasts. Three different sources of cytoplasm (FU27, FU58 and FU85) were used to develop male-sterile A-lines. These three cytoplasm sources were combined with three B. napus B-lines including MillenniUM03, Red River 1826 and UM499. Hybrids were produced using two different ogu INRA restorers to compare the impact of the three cytoplasm sources. Hybrid evaluation trials were conducted in 2012 at three locations within Manitoba. The three cytoplasms had no effect on flowering date, lodging, maturity, oil content or protein content within the Red River 1826 B-line background. Similar results were found within the UM499 B-line background. However, cytoplasm source did have a significant effect on seed yield. At three locations in 2012, the FU85 cytoplasm mean yield was 1681 kg/ha, while the FU58 and FU27 yield was 1555 and 1498 kg/ha, respectively. A similar trend was also seen in the UM499 B-line background; however, the yield differences were not significant. Experiments are being repeated in 2013 and the implications of these results will be discussed.