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Functional Annotation Of The Transcriptome Of TALL Fescue In Response To WATER Stress.

Monday, November 4, 2013: 8:20 AM
Tampa Convention Center, Room 33, Third Floor

Randy D. Dinkins1, Christopher Schardl2 and Padmaja Nagabhyru2, (1)USDA-ARS, Lexington, KY
(2)Department of Plant Pathology, University of Kentucky, Lexington, KY
Tall fescue (Lolium arundinaceum) plants symbiotic with the endophytic fungus, Neotyphodium coenophialum (E+), have better survivability and persistence under stressful conditions, especially under drought stress, than plants lacking the endophyte (E-). To understand more about the grass-endophyte interactions and how endophyte affects the host plant physiology and gene expression, we conducted a time course water stress experiment in the greenhouse using two genetically identical E+ and E- clone pairs of tall fescue. RNA-Seq analysis was done comparing plants on Day 2 stress to control plants.  Analysis was done to compare four tissue types (leaves, pseudostem, crown and root), stress vs non-stressed and presence/absence of the endophyte.  Genes in biotic and abiotic stress response pathways were differentially expressed, as were genes in the jasmonic acid and indoditerpene biosynthetic pathway under stress conditions.
See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: General Genomics, Molecular Genetics & Biotechnology: I

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