Zinc (Zn) deficiency is a major human health concern worldwide. Increasing the contents of nutrients (e.g. Zn) or other compounds in staple crops is an alternative to alleviate micronutrient deficiency, a complementary solution called biofortification. Among the strategies used for biofortification purposes, genetic selection and agronomic intervention are the main ones. One of the most important crops that have been used for Zn biofortification purposes is cassava. This crop is a staple crop in most of the tropical countries, especially the developing ones, which are the most affected by malnutrition and Zn deficiency. The nutritional quality of the cassava root is not sufficient to meet all dietary needs. Thus, more studies on biofortification of this crop can lead to an improvement of its nutritional quality. This study investigated Zn contents in cassava clones enriched with b-carotene and lycopene cultivated under different areas and soil managements in order to evaluate the influence of genotypic variation and agronomic intervention on Zn status in the plant. The Zn content in cassava tubers ranged from 3.6 to 24 mg kg^-1.  The clones 26 and 273 (b-carotene enriched clones) presented highest Zn content in 7 of the 16 studied areas and clone 341 (lycopene enriched clones) presented highest amounts in 3 of the 7 studied areas. Highest values were found in cassava accessions cultivated in an area receiving application of animal manure, which showed high soil Zn content (76 mg kg^-1). These results show that combining genetic and agronomic managements is a good strategy to biofortification purposes.