Forests play an important role in maintaining the hydrologic balance of an area by affecting the flows and storages in surface and subsurface water bodies. Despite their role in the overall hydrological cycle, however, studies on how the forest management strategies affect various components of regional water balance are generally lacking. In this study, we use the soil water balance approach to estimate regional water yields under a range of forest management regimes across Florida. Using the in-situ soil-moisture and groundwater level measurements, stand-scale evapotranspiration (ET) are estimated for forests under different growth stages, understory management, and burning strategies. These ET estimates, combined with the annual rainfall data are then used to derive regional water yields for each management regime. The management-water yield relations developed from these analyses will be critical in guiding the future forest management strategies that are necessary for sustaining the ground and surface water resources in the southeastern US.