Soils have different levels of carbon content or organic matter based on where they are located and how they are formed. This study looks to see if there is a difference in the absorption of estradiol to soil particulate based on carbon content and if the soil carbon has any effect in filtering of hormonal runoff. Carbon partitioning coefficients (K_oc values) were determined for 17β-Estradiol in the soil samples collected and were characterized using High Pressure Liquid Chromatography coupled with Mass Spectroscopy. Analysis of the data uses a Chi square test to determine if there is a difference between similar soils. Additionally, locations of the samples taken were used to determine a possible buffer recommendation along riparian areas.