Plant Transformation Services.

University of Missouri (MU) Plant Transformation Core Facility was established in 2000. The key mission of the Facility is to enhance both basic and applied plant biology research by providing plant transformation services and advancing transgenic technologies. Since 2000, the Facility has been providing state-of-the-art plant transformation services. The services are on fees for cost recovery only, not for profit. The facility staff is dedicated to providing various types of transformation services with a focus on maize (Zea mays), soybean (Glycine max), switchgrass (Panicum virgatum), sorghum (Sorghum bicolor), wheat (Triticum aestivum), and model plant species. The service categories include both standard and customized transformation. Transformation systems for all crops utilize Agrobacterium-mediated approaches and somatic embryogenesis processes except for soybean. The Agrobacterium-mediated cot-node transformation system coupled with organogenesis regime is employed for soybean transformation. Research activities are geared towards developing high-throughput transformation systems, effective small RNA-mediated gene silencing, gene stacking through coordinated transgene expression, and precise genome modifications to meet the needs of crop improvement and genome discoveries. More details on the facility operations and experiences as well as its impact on research collaborations and funding opportunities will be discussed wherever appropriate during the presentation. Readers should find out more about MU Plant Transformation Core Facility at http://plantsci.missouri.edu/muptcf/, email to Zhanyuan J. Zhang at zhangzh@missouri.edu, or call facility office at 573-882-6922 for specific service questions.