Animal agriculture appears to contribute to the spread of antibiotic resistance genes, but few studies have quantified gene transport in agricultural fields. The transport of tylosin, tylosin-resistance genes (erm B, F, A) and tylosin-resistant Enterococcus were measured in tile drainage water from plots treated with manure from tylosin-treated swine.  Enterococcus measured after fall manure application reached 700 cells/g soil, but tylosin-resistant Enterococcus accounted for less than 10% of the total soil population.  Enterococcus soil populations declined over the winter.  Tylosin-resistant bacteria were detected in tile drainage water only in the early spring and tylosin was sporadically detected in drainage water at concentrations below 1 ng/L. The abundance of erm genes was measured by qPCR and those results will be discussed.