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188-2 SCARs Markers Derived From RAPD for Cultivar Identification in Perilla Frutescens.

Poster Number 137

See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Use of Molecular Tools to Enhance Breeding Efforts
Tuesday, November 2, 2010
Long Beach Convention Center, Exhibit Hall BC, Lower Level
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Myoung-Hee Lee1, Tae Joung Ha2, Ki-Woong Yang2, Suk-Bok Pae2, Chung-Dong Hwang2, Chang-Hwan Park2, Kang-Bo Shim2, Chan-Sik Jung2 and Keum-Yong Park2, (1)Rural Development Administration, Milyang, South Korea
(2)National Institute of Crop Science, Rural Development Administration, Milyang, South Korea
Random amplified polymorphic DNA (RAPD) is widely used technique for genetic fingerprinting, but the practical applications of such markers are enhanced by sequencing the RAPD products and converting them more specific SCAR (sequence-characterized amplified region) markers. The objective of the present study was to generate SCARs marker for identification of Perilla frutescens cultivars. Also, this study could offer the derivation of more reliable PCR markers. Seven of the RAPD markers were wholly sequenced and new sequence-specific primers were synthesized for use under more stringent amplification conditions. The positive identification SCAR markers for 30 Perilla frutescens cultivars were obtained 7 specific primer pairs.
See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Use of Molecular Tools to Enhance Breeding Efforts
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