Managing Global Resources for a Secure Future

2017 Annual Meeting | Oct. 22-25 | Tampa, FL

32-1 Microplate Format Assay of Soil Enzymes and Method Standardization.

See more from this Division: SSSA Division: Soil Chemistry
See more from this Session: Soil Enzymes: Methods of Analyses and Mechanisms Oral (includes student competition)

Monday, October 23, 2017: 8:05 AM
Marriott Tampa Waterside, Room 12

Shiping Deng, Plant and Soil Sciences, Oklahoma State University, Stillwater, OK and Richard P. Dick, 406C Kottman Hall, 2021 Coffey Road, Ohio State University, Columbus, OH
Abstract:
In many studies, there is a need for simultaneous analysis of multiple soil enzyme activities within a limited timeframe following collection of a relatively small quantity of soil samples. Such needs could be fulfilled by microplate format enzyme assays. However, there are some challenges and inconsistency employed in these assays; which were largely owe to the complex nature of the soil system and sometimes variations in assay approaches from study to study. Comparison and standardization of methods and assay conditions are of paramount importance to support valid and meaningful data comparison and interpretation, especially when engaging evaluations across systems and studies. This presentation will provide a comprehensive overview of commonly used assay methods with the focus on microplate format assays.

See more from this Division: SSSA Division: Soil Chemistry
See more from this Session: Soil Enzymes: Methods of Analyses and Mechanisms Oral (includes student competition)

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