Discovering Pest Resistances in Primary Synthetics Using Genotyping-By-Sequencing and Their Application in Winter Wheat Breeding.

Primary synthetic wheat has been confirmed as a reservoir of genes for tolerances to various biotic and abiotic stresses in wheat. It has been intensively used in spring wheat breeding, especially for drought and heat tolerances. In this study, we presented the results of 128 primary synthetic wheat lines for their resistances to greenbug, Hessian fly, and wheat curl mite. New sources of resistance are being identified by comparing the linked single nucleotide polymorphisms from genotyping-by-sequencing and known genes based on their chromosome locations. The utilization of these synthetic lines to develop adapted or elite winter wheat lines for high yield potential is discussed.