Sunday, 9 July 2006

Priming Effects Induced by Increasing Amounts of Glucose and Different Sources of CO2 Efflux from Soil Revealed by 14C Labelling and 13C Natural Abundance.

Katja Schneckenberger1, Dmitriy Demin2, and Yakov Kuzyakov1. (1) Univ of Hohenheim, 1 Institute of Soil Science and Land Evaluation, Stuttgart, Germany, (2) Puschino State Univ, Laboratory Functional Ecology, Puschino, Russia

Addition of easily available organic substances (e.g. glucose) to soil can result in a change in mineralization of native soil organic carbon (SOC), termed as priming effect (PE). In C and N dynamics models and C studies, PE are not considered, even though very low amounts of added substances can cause considerable PE. The effects of the amounts of the added substances inducing PE are not investigated well. Also little is known about the origin of the additionally mineralised carbon (C), although it is proposed that this C is released from not stabilized SOC pools. So, the aims of our study were to investigate the effect of strongly increasing glucose amounts on 1) its mineralization and 2) the induced PE on native SOC. Additionally, application of 14C labelled substrate (glucose) to a soil with C3/C4 vegetation changes before 10 years and so using 13C natural abundance should allow to differentiate between PE on old SOC and new C incorporated into SOC during the last 10 years.

Samples of the Ah of a loamy soil 1) from a field cultivated with Miscanthus x giganteus (a perennial C4 plant) for the last 10 years and 2) from a neighboured plot covered with C3 grassland without vegetation changes were used for the incubation experiment (T= 18 C, 60% WHC, 22 days). Increasing glucose amounts (0.25, 2.5, 25, and 250 g glucose-C g-1 soil) were added to the soil as aqueous solution. Each replicate received 14C labelled glucose with an activity of 243 Bq g-1 soil and nutrient solution. Evolved CO2 was trapped in 1 M NaOH solution, where 1) the amount of total CO2 was measured by titration, 2) the amount of glucose derived CO2 was measured as 14C activity by scintillation counting and 3) the d13C values were measured as SrCO3 on a mass spectrometer. So, combining 14C and d13C measurements allowed partitioning of evolved CO2 into three sources: 1) glucose-derived CO2, 2) C3-derived CO2 from old SOC and 3) C4-derived CO2 from new, plant-derived and less stabilized C. Amount and 14C activity of microbial biomass were determined 5 times during the incubation.

With increasing glucose amounts, the proportion of glucose mineralised during the 22 days increased significantly from 29% of added 14C after addition of 0.25 g glucose-C g-1 soil to 44% after addition of 250 g glucose-C g-1 soil. The addition of glucose induced an extra mineralization of SOC from 87 g C g-1 soil (0.25 g glucose-C g-1 soil) up to 174 g C g-1 (250 g glucose-C g-1 soil) during the 22 days. So, the addition of increasing glucose amounts induced higher PE. They increased from 21% of control after addition of the smallest amount of glucose to 43% after addition of the highest amount of glucose.

Before glucose addition, 68% of evolved CO2 was derived from the new C, which was sequestered in the soil during the last 10 years. After the glucose addition, less new C was evolved in treatments with glucose addition (21-28% of total evolved C) than in the control treatment (40% of total evolved C). So, the addition of higher glucose amounts caused greater decreases in mineralization of new C accompanied by greater amounts of additionally mineralised old C. So, we conclude, that during priming effect induced by addition of easily available C source, the additionally mineralised C originated from old, better stabilized SOC.

Combining different methods using stable and radioactive C isotopes is a useful tool for studying the sources of extra mineralised C after addition of easily available substances to soil. This is important for implying PE in C models and investigations of C turnover in soil.

Keywords: SOC, priming effects, glucose, 14C, natural 13C abundance.


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