Evaluation of Different Enzyme-Linked Immunosorbent Assay Test Kits for Detection of Wheat Spindle Streak Mosaic Virus.

Wheat spindle streak mosaic (WSSM), which is caused by Wheat spindle streak mosaic virus (WSSMV), is an important wheat disease that causes significant yield reductions. Over the last 30 years, losses have been minimized by incorporating genetic resistance to this disease into wheat varieties. Programs to identify WSSM resistance rely on visual evaluation of symptoms confirmed using the enzyme-linked immunosorbent assay (ELISA) to detect WSSMV in samples.  Every spring, the Plant Disease and Insect Diagnostic Laboratory at Oklahoma State University performs a wheat survey using ELISA to detect the presence of viruses, including WSSMV. Previously, the test kits used were from Agdia[HB1]  (Elkhart, IN); the purpose of this study was to compare the effectiveness and accuracy of Agdia against two other brands—AC Diagnostics (Fayetteville, AR) and Neogen[HB2]  (Auchincruive, Ayr, Scotland, UK). 

Wheat test plots were planted Oct 8, 2010 in a field known to have WSSMV. The wheat was visually rated for symptoms of WSSM on March 11 and 16, 2011 prior to collecting foliar samples on March 16 for ELISA testing. Samples were harvested by hand, collecting representative leaves to be tested. Twenty-two samples and three known control samples were tested using the three brands.

AC Diagnostics test kits did not detect WSSMV even after repeated testing. Neogen test kits accurately differentiated between WSSM resistant and susceptible lines.  Agdia test kits differentiated between resistant and susceptible lines but also had a high rate of false positives. Subsequently, Agdia was contacted and modified one of its reagents to improve the accuracy of their test, eliminating the problem of false positives.  As a result,  Agdia was the preferred test kit for several reasons, including accuracy, lower cost, ease of use, and availability.  Following this modification, Agdia test kits were used in the spring 2012 wheat disease survey with satisfactory results.

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 [HB1]In parentheses, insert the address of the Company = (Elkhart, IN)

 [HB2]Not sure of this one – you need to check with Jen).

See more from this Division: Students of Agronomy, Soils and Environmental Sciences (SASES)
See more from this Session: Symposium-- National Student Research Symposium Poster Contest

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